Table 1.
Quantification of structural parameters based on 3-D reconstructions of single WT RGC arbors in the dLGN in WT and β2−/− mice at multiple developmental time points
| P4 | P8/P9 | P14/P15 | ||||
|---|---|---|---|---|---|---|
| Number of reconstructed arbors | WT | 10 | 7 | 9 | ||
| β2−/− | 9 | 6 | 6 | |||
| Total length (μm) | WT | 498.70 ± 186.30 | # | 984.36 ± 154.85 | * | 3627.01 ± 842.37 |
| * | ||||||
| β2−/− | 689.16 ± 183.51 | ** | 2216.63 ± 467.06 | 4255.63 ± 1101.23 | ||
| Number of branch points | WT | 2.70 ± 1.50 | *** | 14.14 ± 2.24 | * | 60.89 ± 17.47 |
| β2−/− | 7.11 ± 3.15 | 16.17 ± 4.47 | * | 68.50 ± 23.30 | ||
| Average branch length (μm) | WT | 184.91 ± 42.50 | * | 66.64 ± 6.25 | 65.47 ± 8.65 | |
| ** | ||||||
| β2−/− | 128.86 ± 26.65 | 136.10 ± 17.35 | ** | 68.25 ± 8.37 | ||
| Arbor area (μm2) (coronal plane) | WT | 4787.21 ± 1542.14 | 7507.60 ± 1367.55 | ## | 15,491.57 ± 3263.96 | |
| ### | ||||||
| β2−/− | 5041.15 ± 1190.20 | ** | 12,374.90 ± 2367.78 | 19,417.90 ± 3995.77 | ||
| Arbor density (coronal plane) | WT | 0.15 ± 0.01 | ** | 0.19 ± 0.01 | ** | 0.27 ± 0.02 |
| ** | # | |||||
| β2−/− | 0.18 ± 0.01 | ** | 0.24 ± 0.02 | 0.26 ± 0.02 | ||
| Arbor area (μm2) (sagittal plane) | WT | 3551.08 ± 1215.81 | 5703.62 ± 1331.42 | * | 12,673.87 ± 2282.74 | |
| ** | ## | |||||
| β2−/− | 5886.62 ± 1353.09 | ** | 14,373.75 ± 1895.13 | 21,311.53 ± 4511.34 | ||
| Arbor density (sagittal plane) | WT | 0.18 ± 0.01 | *** | 0.25 ± 0.01 | * | 0.32 ± 0.02 |
| # | ||||||
| β2−/− | 0.18 ± 0.01 | ** | 0.22 ± 0.02 | 0.26 ± 0.02 |
Mean ± SEM of various structural parameters for WT and β2−/− retinogeniculate arbors at different ages. Asterisks between columns and rows identify statistical difference between ages and genotypes:
*p < 0.05;
**p < 0.01;
***p < 0.001. For completion, all p values < 0.1 are reported. Number signs indicate p values for indicated age and genotype groups:
#p = 0.07;
##p = 0.06;
###p = 0.09.