Table 3.
Quantification of structural parameters based on 3-D reconstructions of single contralateral and ipsilateral WT RGC arbors in the dLGN at multiple developmental time points
P4 | P8/P9 | P14/P15 | ||||
---|---|---|---|---|---|---|
Number of reconstructed arbors | Contralateral | 10 | 7 | 9 | ||
Ipsilateral | 6 | 4 | 4 | |||
Total length (μm) | Contralateral | 498.70 ± 186.30 | # | 984.36 ± 154.85 | * | 3627.01 ± 842.37 |
* | ||||||
Ipsilateral | 852.93 ± 157.78 | * | 5821.28 ± 2433.61 | 5364.93 ± 1533.14 | ||
Number of branch points | Contralateral | 2.70 ± 1.50 | *** | 14.14 ± 2.24 | * | 60.89 ± 17.47 |
* | * | |||||
Ipsilateral | 7.67 ± 1.31 | * | 117.75 ± 58.79 | 76.50 ± 13.92 | ||
Average branch length (μm) | Contralateral | 184.91 ± 42.50 | * | 66.64 ± 6.25 | 65.47 ± 8.65 | |
Ipsilateral | 95.85 ± 8.91 | 72.37 ± 16.49 | 74.62 ± 13.92 | |||
Arbor area (μm2) (coronal plane) | Contralateral | 4787.21 ± 1542.14 | 7507.60 ± 1367.55 | ## | 15,491.57 ± 3263.96 | |
* | ||||||
Ipsilateral | 7606.09 ± 1503.82 | 20,366.27 ± 5039.91 | 24,767.69 ± 4905.53 | |||
Arbor density (coronal plane) | Contralateral | 0.15 ± 0.01 | ** | 0.19 ± 0.01 | ** | 0.27 ± 0.02 |
*** | * | |||||
Ipsilateral | 0.20 ± 0.01 | * | 0.30 ± 0.05 | 0.29 ± 0.01 | ||
Arbor area (μm2) (sagittal plane) | Contralateral | 3551.08 ± 1215.81 | 5703.62 ± 1331.42 | * | 12,673.87 ± 2282.74 | |
# | * | * | ||||
Ipsilateral | 6282.46 ± 1066.68 | * | 20,381.20 ± 1066.68 | 27,575.87 ± 8775.13 | ||
Arbor density (sagittal plane) | Contralateral | 0.18 ± 0.01 | *** | 0.25 ± 0.01 | * | 0.32 ± 0.02 |
** | ## | * | ||||
Ipsilateral | 0.22 ± 0.01 | * | 0.31 ± 0.04 | 0.29 ± 0.02 |
Mean ± SEM of various structural parameters for WT contralateral and ipsilateral retinogeniculate arbors at different ages. Asterisks between columns and rows identify statistically significant difference between ages and genotypes:
*p < 0.05;
**p < 0.01;
***p < 0.001. For completion, all p values < 0.1 are reported.
#p = 0.07;
##p = 0.06.