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. Author manuscript; available in PMC: 2011 Mar 18.
Published in final edited form as: ACS Chem Biol. 2007 Aug 17;2(8):561–571. doi: 10.1021/cb700110z

Figure 4.

Figure 4

Microarray analysis of gene expression. a) Divisive clustering analysis over all interrogated transcripts for DFO-induced cells: HIF-1α siRNA, R; echinomycin (100 nM), E; and polyamides 1 (1 μM). b) Agglomerative clustering analysis over all 297 transcripts induced by DFO ≥4-fold (p ≤ 0.01). Of this transcript set, HIF-1α siRNA inhibited 244, echinomycin inhibited 263, and polyamide 1 inhibited 69 by ≥2-fold (p ≤ 0.01). c) Effects of the indicated treatments on a panel of genes previously characterized as direct targets of HIF-1 and also induced by DFO at least 1.5-fold (p ≤ 0.01) in this experiment. Treatments reported are an error-weighted average from three experiments. d) Venn diagrams representing transcripts commonly down- and up-regulated (|fold-change| ≥ 2.0, p ≤ 0.01) by 1 and HIF-1α siRNA, by 1 and echinomycin, and by HIF-1α siRNA and echinomycin. Numbers inside the intersections represent transcripts affected by both treatments.