Table 1.

Primer sequences and annealing temperatures for PCR

Gene	Ref. Seq	Exon	Function	Forward Primer	Reverse Primer	Annealing Temperature °C
WISP3	NM_003880	4	Cell attachment domain.	GATAGAGTGATAAATTAGACCATCGGCT	CATTGGTCACCCTGTTAGATATTCC	55
CTNNB1	NM_001904	3	GSK3b regulatory domain	ATGGAACCAGACAGAAAAGCGGC	GCTACTTGTTCTTGAGTGAAG	58
APC	NM_000038	15a	b-Catenin regulatory domain	CAGACTTATTGTGTAGAAGA	CTCCTGAAGAAAATTCAACA	52
APC		15b	b-Catenin regulatory domain	AGGGTTCTAGTTTATCTTCA	TCTGCTTGGTGGCATGGTTT	52
APC		15c	b-Catenin regulatory domain	GGCATTATAAGCCCCAGTGA	TGGCTCATCGAGGCTCAGAG	52
APC		15d	b-Catenin regulatory domain	ACTCCAGATGGATTTTCTTG	GGCTGGCTTTTTTGCTTTAC	52
AXIN1	NM_003502	2a	APC binding	CGTCATCGTGAGTCTTGTCT	TCAGCCCACTTCAAGTATGG	55
AXIN1		2b	APC binding	AAGGTGAGACTTCGACGG	ATAGTGGCCTGGATTTCGGT	55
AXIN1		2c	APC binding	AACAATGGCATCGTGTCCCG	TGTCTCCAGGAGCAGCTTCT	55
AXIN1		2d	APC binding	CCTGCCGACCTTAAATGAAG	GGACATCCGGTGTGGGTTAA	55
AXIN1		3	GSK3b binding	ACAGGTGGAGATGTTGGTC	ACACATTGCTGTCCTCAAGG	55
AXIN1		4	GSK3b binding	ATCACCTGTGTGCACGTGT	ACTGGCCACTTGCAGATG	55
AXIN1		5	GSK3b binding	AGCACACGCTTTCCCTTCA	CCCATGAAGAAGCATCAGGAC	55
AXIN1		6a	b-Catenin binding	TGGGTCAGGCCTGATGCTTTT	ACTGGCATCTTGGCCACGT	55
AXIN1		6b	b-Catenin binding	AGCATCCTGGACGAGCACGT	TGCAGAAGGCCAGAACCT	55
AXIN2	NM_004655	7	DSH Binding	CAAAGCACAAAAAAGGCCTAC	GATTCCTGTCCCTCTGCTGAC	60