Table 3.

Summary of β-catenin immunodetection and sequence analysis of Wnt pathway genes in Metaplastic carcinomas of the breast

			β-catenin IH (a)			CTNNB1 mutation			APC mutation			WISP3 mutation			Histologic component harboring the mutation
Case	Age	Grade	N	C	M	Nucleotide	Amino acid	Codon	Nucleotide	Amino acid	Codon	Nucleotide	Amino acid	Codon
1	46	2	pos	pos	neg				A-T	K-Stop	1317	del G	Frameshift	1141	spindle (APC) and glandular (WISP3)
2	52	2	neg	pos	neg
3	70	3	pos	pos	neg	TCT-TGT	Ser-Cys	33							spindle
4	67	2	pos	neg	neg	TCT-TAT	Ser-Tyr	37	A-T	K-Stop	1317				spindle (APC) and glandular (CTNNB1 )
5	71	2	pos	pos	neg
6	58	3	pos	pos	neg	ACA-AGA	Thr-Arg	42							glandular
7	87	2	pos	pos	neg	TCT-TGT	Ser-Cys	33							spindle
8	48	3	pos	pos	neg
9	50	3	pos	pos	neg
10	71	3	pos	pos	neg							del G	Frameshift	1141	squamous and glandular
11	40	3	pos	pos	weak
12	58	3	neg	neg	weak	ACA-AGA	Thr-Arg	42							glandular
13	33	3	neg	neg	weak
14	72	3	neg	neg	pos
15	35	3	neg	pos	pos
16	50	3	pos	pos	weak
17	50	3	pos	pos	pos
18	42	3	neg	pos	pos							del G	Frameshift	1141	chondroid
19	60	3	neg	neg	weak							del G	Frameshift	1141	glandular
20	67	3	neg	pos	weak
21	36	2	pos	pos	neg
22	48	2	neg	pos	red
23	40	3	pos	pos	weak
24	75	3	neg	pos	neg
25	89	2	neg	yes	weak	TCT-TGT	Ser-Cys	33				del G	Frameshift	1141	squamous
26	NA	3	neg	neg	neg	TCT-ACT	Ser-Thr	33
27	54	3	pos	neg	neg