KLF6 expression in OSE brushings versus select housekeeping genes. (A) KLF6 microarray signal intensity values were averaged for 10 OSE brushings and compared to the average expression levels of 5 housekeeping genes: GUSB, ACTA2, ACTB, PPIH, and PPIA. The KLF6 expression level was comparable to the housekeeping gene levels, and this indicates that KLF6 is reliably expressed in ovarian epithelial cells. (B) KLF6 expression in 76 stage III EOC samples versus KLF6 expression in OSE brushings. The KLF6 expression in each microdissected EOC sample was compared to the average expression in all 10 OSE samples. The fold changes were normalized to zero. Abbreviations: ACTA2, actin alpha 2 smooth muscle aorta; ACTB, actin beta; EOC, epithelial ovarian cancer; GUSB, glucuronidase beta; HOSE, human ovarian surface epithelial; KLF6, Kruppel-like factor 6; OSE, ovarian surface epithelium; PPIA, peptidylprolyl isomerase A (cyclophilin A); PPIH, peptidylprolyl isomerase H (cyclophilin H).