Figure 3. Tetramer formation - dependence on imidazol concentration and pH.

Analytical gel filtration on a Superdex 200 column was used to estimate the fraction of S100B in tetrameric state after triggering experiments starting with 20 mg/ml highly pure S100B dimer in 1 mM EDTA, 1 mM DTT and 0.25 M NaCl with different concentrations of imidazol at pH 7.0 (A) or with 90 mM imidazol at different pH values (B).