Figure 6. Effects of LPS stimulation in GFAP-IκBαAA and GFAP-IκBαAA/SOD1G93A mice.
(A) Effect of intraperitoneally LPS injection on survival of 10 weeks old non transgenic (-) and transgenic (Tg-A and Tg-D) mice. N = 20 mice/group (10 males and 10 females). (B) Effect of 3 h LPS treatment on iNOS, IL-1β, TNFα, Fas mRNA expression in tissues as brain or spinal cord from GFAP-IκBα-AA mice as determined by semi-quantitave PCR. Results obtained with tissues from stimulated non transgenic mice are defined as 100% and compared with their corresponding results from stimulated transgenic samples. Data are normalized to β-actin. *p<0.05 **p<0.01. Results represent the mean +/−SEM of three animals/group. (C) Western blot analysis on spinal cord protein extract from non transgenic (-) and GFAP-IκBαAA transgenic mice (TgA) using antibodies against TRL2, TRL4 and β-actin.