Figure 3. Kinetics of GFP production by the L. biflexa reporter strains (P41G, PAG and P2G) following induction by NaCl to a physiological level.

Cultures of the reporter strains containing a single-copy transcriptional reporter and either the wild-type lipL41 (A), ligA (B) or sph2 (C) promoter regions were induced with ∼300 mosmol NaCl (physiological level). An induced non-transformed L. biflexa serovar Patoc str. Patoc 1 (P-24) was induced for 24 h as a control. The uninduced reporter strains (NI-24) were included as additional controls. Transcriptional activity was presented as the mean ± standard error (bars). Fluorescence levels from triplicate samples of each culture were standardized according to an OD₄₂₀ 0.5 and are expressed as arbitrary fluorescence units. Data from a representative significant study are shown.