Figure 4. Kinetics of GFP production by the L. biflexa reporter strains (P41G, PAG and P2G) after a temperature upshift to 37°C.

Cultures of the reporter strains P41G (A), PAG (B) or P2G (C) were induced by temperature upshift from 30°C to 37°C (physiological temperature). L. biflexa serovar Patoc str. Patoc 1 (P-24) induced for 24 h and uninduced reporter strains grown (NI-24) were included as controls. Transcriptional activity was measured for 24 h and presented as the mean ± standard error (bars). Fluorescence from triplicate samples of each culture were standardized according to an OD₄₂₀ 0.5 and are expressed as arbitrary fluorescence units. Data from a representative significant study are shown.