Table 2. Comparison among top fluorescence intensities and protein expression levels reached by the strains in study.

Treatments	Reporter strain
	P41G	PAG	P2G
Physiological osmolarity	1.11	1.48	1.03
Temperature upshift to 37°C	1.46	1.50	1.26
Physiological osmolarity/Temperature upshift	1.54	2.59*	1.62
Physiological osmolarity/Temperature upshift/pH 6.7	1.58	2.08*	1.96†
Physiological osmolarity/Temperature upshift/Spermine	1.58	2.74*	2.12*

*Corresponds to signal intensity at least 2-fold higher than that observed for the non-induced control.

^†Corresponds to the border line of 2-fold higher signal intensity, compared to the non-induced control.

NA - Not applicable.

^‡Relative to the parental virulent strain, L. interrogans serovar Copenhageni str. Fiocruz L1-130 strain.