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. 2011 Mar 18;6(3):e17753. doi: 10.1371/journal.pone.0017753

Figure 4. qRTPCR validation.

Figure 4

(A, B) qRTPCR relative expression levels of genes upregulated in PT (A) and PFO (B) using the ΔΔCT relative quantification method, normalized to the PFO-17GW-right sample. and to the housekeeping gene GAPDH. (C, D) Comparison of the PFO VS PT expression ratios in microarray and qPCR results. Mean of the PFO 17GW/PT17GW and PFO19GW/PT19GW ratios for the qPCR and microarrays results for genes upregulated in PT (C) and PFO (D).