Figure 5. Nodal signalling is affected in Hexd^act/+ embryos.

(A) Nodal expression is not restricted to the posterior in Hexd^act/+ embryos at 6.5dpc (n = 8/28). (B) Bmp2 expression is expanded in the anterior visceral endoderm (red arrow) of Hexd^act/+ embryos; n = 7/15. (C) Proximal expression of Wnt3 in Hexd^act/+ embryos; n = 3/6. (D) Culture with BMP2 leads to ectopic Nodal^LacZ/+ expression (controls that are posterior n = 10/10; BMP2 treated posterior n = 1/8). (E) β-galactosidase expression in Nodal^LacZ/+and Hexd^act/+;Nodal^LacZ/+ embryos at 6.5dpc (weak and proximal expression n = 6/8). (F–G) T and Fgf8 expression in control and Hexd^act/+;Nodal^LacZ/+ embryos at 7.5dpc. Hexd^act/+ embryos with reduced expression T n = 7/17 and Fgf8 n = 6/11. Scale bar 90 µm.