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. 2011 Mar 18;6(3):e17615. doi: 10.1371/journal.pone.0017615

Figure 2. Immunoblot analyses of soluble Microcystis protein extracts using a microcystin-specific antibody.

Figure 2

A) Protein extracts of the ΔmcyH, ΔmcyB mutants and wild type. B) Protein extracts of ΔmcyB mutant prior and after addition of microcystin-LR and DTNB pre-treatment (left). Protein extracts of ΔmcyB mutant with addition of microcystin-LR or cysteamin-linked microcystin-LR MC* (right). C) Modified microcystin variant with a cysteamin group attached to the N-methyldehydroalanine moiety.