Table 3. Substrate specificity of PaMpl.

Substrate	k (min−1)
L-Ala-γ-D-Glu-meso-A2pm	260±20a
L-Ala-γ-D-Glu-meso-A2pm-D-Ala	5.9±0.6
L-Ala-γ-D-Glu-meso-A2pm-D-Ala-D-Ala	5.3±0.6
L-Ala	0.7±0.1
L-Ala-D-Glu	1.4±0.2
L-Ala-γ-D-Glu-L-Lys	0.25±0.05
L-Ala-γ-D-Glu-L-Lys-D-Ala	0.10±0.03
L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala	NDb

The k (v/E) values were determined with 0.25 mM of meso-A₂pm-containing peptides, 4 mM of L-lysine-containing peptides, and 15 mM of L-Ala or L-Ala-D-Glu.

^aApparent k _cat value.

^bND, no detectable product formation under the experimental conditions used.