Table 1.

Germination and outgrowth of B. anthracis spores as a function of cell culture medium in the presence or absence of FBS^a.

			outgrowthe
mediumb	FBSc	germinationd	1 h	4 h
DMEM	-	-	-	-
	+	+	+	+
RPMI	-	-	-	-
	+	+	+	+
MEMα	-	+	+	+
	+	+	+	+
MEM	-	-	-	-
	+	+	+	+
AMEM	-	-	-	-
	+	+	+	+
EMEM	-	-	-	-
	+	+	+	+
BME	-	-	-	-
	+	+	+	+
CIM	-	+	+	+
	+	+	+	+
F-12	-	-	-	-
	+	+	+	+
M5A	-	+	+	+
	+	+	+	+
BHI	-	+	+	+
LB	-	+	+	+
AAf	-	+	-	-

^a Three independent experiments were performed with three different spore preparations, each conducted in triplicate.

^b Spores prepared from B. anthracis Sterne 7702 were incubated in the indicated medium.

^c Indicates the presence (+) or absence (-) of 10% FBS in the indicated medium.

^d Spores were scored positive (+) for germination if the OD_{600 nm} of the suspended spores decreased by more than 10% after 30 min incubation in the indicated medium.

^e Using DIC microscopy, spores were scored positive (+) for outgrowth if the spores bodies were visibly larger at 1 h, and had developed into vegetative bacteria by 4 h.

^f AA refers to L-alanine and L-inosine (each at 10 mM, in PBS pH 7.2).