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. 2011 Mar 3;11:42. doi: 10.1186/1471-2229-11-42

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Copyright ©2011 Arce et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ATHB1 CTR acts as an activation domain in yeast cells. (A) The complete sequence of ATHB1, a version without the CTR (ATHB1WCT), and the CTR alone (ATH1CT) were fused to the DNA-binding domain of GAL4 (GAL4-BD). The empty vector expressing only the GAL4-BD was used as negative control. (B) A β-galactosidase activity assay. (C) Confirming this results, only the CTR and the complete ATHB1 protein had the transactivation activity required to reverse the auxotrophy to His of the AH109 yeast cells, allowing them to grow in medium lacking this amino acid.