Table 3.
Oligonucletotides used for cloning
| Oligonucleotide name | Sequence | Restriction sites | construct |
|---|---|---|---|
| Transfl | 5'-gCggATCCACCATgTCTTTCAACAAgTA-3' | BamHI | H4-H1 cloning |
| H1CDS-R | 5'-ggggAgCTCTCAATTgAATTgTggTTgTTCC-3' | SacI | H4-H1 cloning |
| H1*H4F | 5'-gTTggAggTgTAAAAAATAgggAgCCAgC-3' | H4-H1 cloning | |
| H1*H4R | 5'-TATTTTTTTAgCACCTCCAACTgATTgAgTAgg-3' | H4-H1 cloning | |
| H4-WCT-R | 5'-CCCgAgTCTCTATTCTTCACCgCTgCCAC-3' | SacI | H4WCT cloning |
| H1-WCT-R | 5'-ggCgAgCTCTCATCCTTCTgTTTCTTTTATgTTgAgg-3' | SacI | H1WCT cloning |
| H1atF | 5'-gggggATCCgCTgATgACTTgCACTggAATggC-3' | BamHI | H1WCT cloning |
| H1qR | 5'-CCAACCATggCCAAAACCCTg-3' | H1 qRT-PCR | |
| H1qF | 5'-ggCCggCAgATCATCAACTTC-3' | H1 qRT-PCR | |
| H4qR | 5'-gCCgAgTCTTAgAACTCCAACCACTTTTg-3' | H4 qRT-PCR | |
| H4qF | 5'-CgCgATCAAAgTCgAggCAgATTg-3' | H4 qRT-PCR | |
| H4quimqF | 5'-CgCgATCAAAgTCgAggCAgATTg-3' | H4-H1 qRT-PCR | |
| H1AsTH4R | 5'-TCCTTCTgTTTCTTTTATgTTgAgg-3' | H4-H1 qRT-PCR | |
| UBCQPCR-R | 5'-CAgTggACTCgTACTTgTTCTTgT-3' | Genomic DNA | |
| UBC9GENOM-F | 5'-gTTTTggAAATgTTgACAggAC-3' | Genomic DNA | |
| ATHB1F | 5' gCggAATTCATggAATCCAATTCgTTTTTC 3' | EcoRI | ATHB1 and ATHB1WCT cloning |
| ATHB1R | 5' gCgggATCCTAAggCCATCCCCAgAAAg 3' | BamH1 | ATHB1 cloning |
| ATHB1WCTR | 5' gCggTCgACTACTCTTgTTTgCCCTgAAgC 3' | Sal1 | ATHB1WCT cloning |
| ATHB1CTF | 5' gCggAATTCCAAgAgACAgCTAATgAACCA 3' | EcoRI | ATHB1 CTR cloning |
Sequence of oligonucleotides used in PCR reactions to perform constructs or cDNA quantifying. In bold, the overlapping sequences for chimerical constructs and underlined the restriction sites used for cloning.