Figure 3.
CD3+CD4+ and CD3+CD8+ IFN-γ responses to vaccine insert as measured by intracellular cytokine staining. Intracellular IFN-γ responses after background subtraction in (A) CD3+CD8+ and (B) CD3+CD4+ cell populations stimulated with 1 pool of peptides spanning the complete NP+M1 vaccine insert. Volunteers in group 3 were tested at weeks 0, 1, and 8. Median % IFN-γ+ within CD3+CD8+ cells at week 1 = .4% and week 8 = .33%; median % IFN-γ+ cells within CD3+CD4+ population at week 1 = .098% and week 8 = .039%.