Figure 3.

CD3⁺CD4⁺ and CD3⁺CD8⁺ IFN-γ responses to vaccine insert as measured by intracellular cytokine staining. Intracellular IFN-γ responses after background subtraction in (A) CD3⁺CD8⁺ and (B) CD3⁺CD4⁺ cell populations stimulated with 1 pool of peptides spanning the complete NP+M1 vaccine insert. Volunteers in group 3 were tested at weeks 0, 1, and 8. Median % IFN-γ+ within CD3⁺CD8⁺ cells at week 1 = .4% and week 8 = .33%; median % IFN-γ+ cells within CD3⁺CD4⁺ population at week 1 = .098% and week 8 = .039%.