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CD34+ HPC were stained with CFSE and cultured either without stroma (A) or in co-culture with MSC (B) in culture medium supplemented either with 10% BC serum or 10% d8 serum. After seven days expression of CD3, CD13, CD19, CD33, CD45, CD56 and CD133 were analyzed by flow cytometry. Representative results for four patients are demonstrated (each of them performed in duplicate).
