Table 1. Efficiency of vertical DNA delivery to F0 individual 4^th instar larva (relative AeSCP-2 mRNA levels vs. rpL8).

	Batch#1 (larvae/48 hr PSI)*		Batch#2 (larvae/72 hr PSI)*
	control	siRNA induced	control	siRNA induced
1	8.77	5.79	4.81	1.52
2	9.19	2.30	5.79	0.55
3	8.00	4.59	6.20	1.45
4	7.13	2.19	9.62	2.83
5	7.29	0.98	4.29	0.31
6	8.77	4.49	7.82	2.05
7	9.19	0.68	7.82	0.17
8	5.79	0.17	5.79	0.10
9	8.77	0.52	5.28	0.03
10	5.28	0.35	1.18**	1.05
11	–	–	7.46	2.41
12	–	–	3.32	0.95
13	–	–	3.48	0.59
14	–	–	2.96	2.30
15	–	–	1.91**	0.19
stages§	Late 3rd	Early 3rd	Early 4th	Late 3rd
mean	7.82	2.21	5.18	1.10
S.D.	1.41	2.06	2.30	0.91
t-test		p = 0.000001		p = 0.000001

*PSI = post siRNA induction: synchronized day1 2^nd instar larvae were heat shocked at 42°C for 3 hour and returned to 26°C for the duration before sample collection. Control =  wild type individuals experienced identical treatment as the siRNA induced groups.

**Newly molt 4^th instar larva.

^§The development stage when samples were collected.