Table 3. Effects of AeSCP-2 siRNA expression on reproduction.
From 30 larvae/batch to 5 days after adult emergence | batch | male | Female | Fed BM | eggs | Eggs/Female | Hatched | % hatch | Fertility* |
Control, started at 2nd instar | #1 | 9 | 10 | 9 | 642 | 71 | 497 | 77.41% | 55 |
#2 | 10 | 10 | 9 | 672 | 67 | 537 | 79.91% | 54 | |
#3 | 9 | 12 | 11 | 772 | 64 | 627 | 81.22% | 52 | |
#4 | 10 | 13 | 13 | 827 | 64 | 665 | 80.41% | 52 | |
Induced siRNA at 2nd instar | #1 | 3 | 14 | 11 | 526 | 48 | 332 | 63.12% | 30 |
#2 | 6 | 11 | 9 | 437 | 40 | 223 | 51.03% | 20 | |
#3 | 5 | 12 | 10 | 495 | 41 | 257 | 51.92% | 21 | |
#4 | 6 | 12 | 10 | 507 | 42 | 261 | 51.48% | 22 | |
Control, started at 4th instar | 10 | 12 | 11 | 809 | 74 | 597 | 73.79% | 55 | |
Induced siRNA at 4th instar | 5 | 11 | 9 | 646 | 72 | 337 | 52.17% | 38 |
*Fertility = viable F1 offspring/female (larvae were countered during 2nd-3rd instar).