Table 4. Primers and oligo sequences for qPCR and AeSCP-2 siRNA.* .
Gene | |
Rpl8 | F: 5′-TACCTGAAGGGAACCGTCAAGCAA-3′ |
R: 5′-ACAATGGTACCTTCGGGCATCAGA-3′ | |
Actin-1 | F: 5′-CCCTGAAGTACCCCAATGAGC-3′ |
R: 5′-CCATGTCATCCCAGTTGGTG-3′ | |
AeSCP-2 | F: 5′- GCTGGTCGAGTCCGACGATGC-3′ |
R: 5′- CAGGGCACCGGTTCCGATGG-3′ | |
siRNA | Sense: 5′-ggaattcGTCAAGCTGGTCGAGTCCGACgggcccGTCGGACTCGACCAGCTTGaagcttggg-3′ |
Antisense: 5′-cccaagcttCAAGCTGGTCGAGTCCGACgggcccGTCGGACTCGACCAGCTTGACgaattcc-3′ |