Skip to main content
. 2011 Mar 18;6(3):e18030. doi: 10.1371/journal.pone.0018030

Table 4. Primers and oligo sequences for qPCR and AeSCP-2 siRNA.* .

Gene
Rpl8 F: 5′-TACCTGAAGGGAACCGTCAAGCAA-3′
R: 5′-ACAATGGTACCTTCGGGCATCAGA-3′
Actin-1 F: 5′-CCCTGAAGTACCCCAATGAGC-3′
R: 5′-CCATGTCATCCCAGTTGGTG-3′
AeSCP-2 F: 5′- GCTGGTCGAGTCCGACGATGC-3′
R: 5′- CAGGGCACCGGTTCCGATGG-3′
siRNA Sense: 5′-ggaattcGTCAAGCTGGTCGAGTCCGACgggcccGTCGGACTCGACCAGCTTGaagcttggg-3′
Antisense: 5′-cccaagcttCAAGCTGGTCGAGTCCGACgggcccGTCGGACTCGACCAGCTTGACgaattcc-3′

*The capital letters represent the sequences of targeted genes; lower case letters represent added DNA sequences for directional cloning or diagnostic restriction digest (underlined lower case letters). AeSCP-2: AY190283; AeRpL8: M99055; AeAct-1: U20287.