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. Author manuscript; available in PMC: 2012 Apr 10.
Published in final edited form as: Virology. 2011 Feb 26;412(2):315–324. doi: 10.1016/j.virol.2011.01.017

Figure 2. Effects of CD63 silencing on HIV-1 replication in human MDMs and U373-MAGI-CCR5 cells.

Figure 2

Figure 2

(A) MDMs (5 × 105 cells/well) or (B) U373-MAGI-CCR5 cells (1 × 105 cells/well) were plated in triplicate in 24-well plates and transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Controls included cells treated with raltegravir (20 mM), untreated cells or cells treated with virus infection only. Forty eight hours post-transfection, cells were infected with HIV-1 SX (m.o.i. =0.02). Supernatants were harvested for p24 detection on day 5 post-infection for U373-MAGI-CCR5 cells, and on day 7 post-infection for MDMs using p24 Capture ELISA kit (ImmunoDiagnostics, Woburn, MA). (C) Cell lysates from MDMs on day 7 post-infection were also harvested for detection of intracellular p24. *P<0.05, **P<0.01, compared with ERBB2IP control group, respectively.