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. Author manuscript; available in PMC: 2012 Apr 10.
Published in final edited form as: Virology. 2011 Feb 26;412(2):315–324. doi: 10.1016/j.virol.2011.01.017

Figure 4. CD63 silencing has no effect on cell-cell fusion.

Figure 4

U373-MAGI-CCR5 target cells (104cells/well) were transfected with CD63-, CD4-, or CCR5-specific siRNAs and ERBB2IP siRNA. For controls, fusion inhibitor T20 (1 μg/ml or 10 μg/ml) was used as well as fusion only conditions containing no siRNA. Forty-eight hours post-transfection U373-MAGI-CCR5 target cells were co-cultured with 2 × 104 of HL2/3 effector cells per well at 37°C for 12 h to allow fusion to occur. Fusion was monitored by assaying for Tat-dependent β-gal reporter gene activation stimulated by HIV-1 Tat from the HL2/3 cells. All experiments were performed in quadruplicate. *P<0.05, **P<0.01, compared with ERBB2IP group.