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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Exp Eye Res. 2011 Feb 12;92(4):251–259. doi: 10.1016/j.exer.2011.02.003

Figure 1.

Figure 1

Y79 cells express Cx46 and thrive under hypoxic conditions. (A) Y79 whole cell lysates probed with rabbit anti-Cx46 antibody and mouse anti-tubulin antibody, demonstrating Cx46 expression in human Y79 retinoblastoma cells. (B) Viability of Y79 cells under normoxia (37°C humidified ambient air, 5% CO2) and hypoxia (37°C humidified 1% oxygen displaced by N2, 5% CO2) was monitored for 7 days and recorded. (C) Total Y79 cell numbers were counted for 7 days under normoxic and hypoxic growth conditions. Data trends from several experiments indicate that Y79 cells express Cx46 and can survive and thrive in hypoxic conditions (error not calculated). Representative experimental data shown, additional data in supplement.