Table 1.
NK cell depletion suppresses IL-6 production by Kupffer cellsa
| Treatment | Cell population | IL-6 (pg/ml) | |
|---|---|---|---|
| Experiment 1 | control | total NPC | 142.6 ± 21.1 |
| Kupffer cell | 1,172.2 ± 167 | ||
| NK cell-depleted | total NPC | 94.8 ± 19.7b | |
| Kupffer cell | 515.2 ± 61.5b | ||
| Experiment 2 | control | total NPC | 126.7 ± 23.7 |
| Kupffer cell | 1,683.3 ± 31.5 | ||
| anti-IFN-γ | total NPC | 61.4 ± 6.8b | |
| Kupffer cell | 743.6 ± 37.9b |
a
Total NPC and purified Kupffer cells were isolated from groups of 5 control, NK cell-depleted (Experiment 1), or anti-IFN-γ-treated [500 μg/mouse i.v 1 hour prior to surgery; control animals received 500 μg normal rat IgG (Experiment 2)] mice at 6 hours post-BDL and cultured at 1×105 cells/well in 96-well tissue culture plates. The culture supernates were collected after an approximate 40-hour incubation period; IL-6 was quantified by ELISA. Data are the mean ± SD pg/ml derived from triplicate wells. Both experiments were performed twice yielding similar results.