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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Dec 15;508(2):198–203. doi: 10.1016/j.abb.2010.12.012

Figure 1. Proteosomal inhibitors block the degradation of p53 in (A) 8B20 and (B) B16F10 melanoma cell lines.

Figure 1

Exponentially growing 8B20 and B16F10 cell lines were either untreated (C: control), or treated with ethanol (V: vehicle control), proteosomal inhibitors (MG132 and LC: clasto-lactacystin-β-lactone) or non-proteosomal inhibitors (E-64 and PMSF) at the indicated concentrations for 6 hours. Cell lysate (100 μg) was loaded on a 12 % polyacrylamide gel and subjected to SDS-PAGE and immunoblot analysis. Membranes were probed with anti-p53 antibody (mouse specific). β-Actin levels are shown as loading controls. The data are one of three representative experiments.