Figure 5.
Effect of different approaches to blocking MDSC expansion on survival to sepsis. Mice were pretreated with either gemcitabine or anti–GR-1 monoclonal antibodies, or antibodies to SDF-1 and subjected to an LD30 cecal ligation and puncture, as described in detail in the Supplemental Methods. Survival to CLP was dramatically reduced in mice treated with either gemcitabine (A, n = 20 in each group) or anti–GR-1 antibodies (C, n = 20 in each group). In surviving animals, there was evidence that the expansion of the MDSC population at 7 d was markedly suppressed (*P < 0.01).