Figure 3.

Comparison of C-terminal domains of PP1 and PP2A and construction of PP1/PP2A chimeras. (A) Alignment of amino acid sequences of human PP1α and bovine PP2A. Conserved amino acids (including homologous substitutions) are boxed, and identical amino acids are indicated with a gray background. Positions of β-strands of PP1 are indicated by gray boxes above the alignment. Amino acids altered in the PP1/2A chimeras are underlined in bold. (B) Construction of PP1/PP2A chimeras. The highly conserved core region of PP1 (residues 41–273) and the corresponding region of PP2A are indicated by light gray and dark gray bars, respectively. N- and C-terminal variable regions of PP1 and PP2A are indicated by white and black bars.