. 2010 Sep 14;40(4):1077–1090. doi: 10.1007/s00726-010-0731-z

Table 1.

Effects of pyruvate (PYR; 1 mM) incubated with whole blood for 10, 60 and 120 min on important free intracellular amino acids (10⁻¹⁶ Mol per PMN-cell; mean ± SD; n = 10), α-ketoglutarate and pyruvate concentrations in PMN (10⁻¹⁷ Mol per PMN-cell; mean ± SD; n = 10)as well as on PMN superoxide anion production [O₂ ⁻; fmol/(PMN min)⁻¹], hydrogen peroxide formation [H₂O₂; fmol/(PMN min)⁻¹] and myeloperoxidase activity [MPO; units/l supernatant]; (mean ± SD; n = 10)

	10 min		60 min		120 min
	Mean	SD	Mean	SD	Mean	SD
Control
Asparagine	0.39	0.10	0.41	0.09	0.37	0.09
Glutamine	3.11	0.77	3.32	0.91	2.90	0.86
Aspartate	2.65	0.73	2.80	0.61	2.56	0.57
Glutamate	5.91	1.16	6.28	1.81	5.79	1.56
Ornithine	0.46	0.08	0.49	0.08	0.45	0.09
Arginine	0.35	0.07	0.37	0.07	0.34	0.08
Citrulline	0.14	0.03	0.14	0.03	0.14	0.03
Serine	2.30	0.44	2.22	0.53	2.07	0.45
Glycine	2.47	0.56	2.35	0.49	2.29	0.58
Alanine	1.94	0.46	1.86	0.46	1.81	0.59
α-Ketoglutarate	1.28	0.30	1.25	0.34	1.17	0.35
PYRUVATE	6.36	1.84	6.14	1.93	5.92	1.56
O₂ ⁻	3.608	0.959	3.484	0.886	3.264	0.773
H₂O₂	1.279	0.316	1.263	0.365	1.198	0.304
MPO	0.584	0.157	0.545	0.190	0.499	0.172
PYR (1 mM)
Asparagine	0.40	0.11	0.53*^#	0.12	0.65*^#	0.17
Glutamine	2.92	0.78	4.62*^#	1.27	5.54*^#	1.79
Aspartate	2.87	0.80	4.27*^#	1.32	5.18*^#	1.84
Glutamate	6.90	1.45	11.66*^#	3.91	13.84*^#	4.32
Ornithine	0.48	0.11	0.58	0.14	0.73*^#	0.24
Arginine	0.36	0.10	0.42	0.11	0.48*^#	0.12
Citrulline	0.13	0.03	0.14	0.04	0.16	0.05
Serine	2.19	0.46	2.52	0.67	3.01*^#	0.66
Glycine	2.22	0.44	2.54	0.59	3.41*^#	0.91
Alanine	2.26	0.57	3.34*^#	0.98	5.05*^#	1.82
α-ketoglutarate	1.34	0.37	2.01*^#	0.57	2.78*^#	0.93
PYRUVATE	7.23	2.29	12.07*^#	3.36	19.31*^#	7.54
O₂ ⁻	4.652	1.195	10.306*^#	3.051	17.091*^#	6.560
H₂O₂	1.565	0.437	3.182*^#	0.846	4.992*^#	1.926
MPO	0.672	0.211	1.208*^#	0.413	1.502*^#	0.738

* p ≤ 0.05 versus control values; # p ≤ 0.05 versus 10 min