Table 2.

Comparison of different segments within OPT proteins using the GAP and IC programs

Comparison	Segment	Protein-1	Amino acids	TMS	Protein-2	Amino acids	TMS	IC/GAP score (SD)	Average score (SD)
1. 1–8 vs. 9–16	AB vs. CD	Spr1	16–241	1–8	Lsa1	358–589	9–16	12.6	12.0
	AB vs. CD	Zma1	51–216	1–4	Chy1	358–505	9–12	11.3
2. 1–4 vs. 5–8	A vs. B	Hso1	41–139	1–3	Sde1	174–270	5–7	11.9	11.3
	A vs. B	Ngo1	45–143	1–3	Sde1	174–270	5–7	10.7
3. 1–4 vs. 9–12	A vs. C	Zma1	51–159	1–3	Chy1	358–455	9–11	12.5	12.2
	A vs. C	Mth1	14–123	1–4	Mgr3	467–577	9–12	11.9
4. 1–4 vs. 13–16	A vs. D	Gze4	139–266	1–2	Sus1	532–662	13–14	12.1	11.9
	A vs. D	Mxa5	54–147	1–3	Ckl1	512–604	13–15	11.8
5. 5–8 vs. 9–12	B vs. C	Sco1	327–427	7–8	Mtu1	366–461	11–12	12.2	11.6
	B vs. C	Sco1	320–435	6–8	Ath5	414–531	10–12	10.9
6. 5–8 vs. 13–16	B vs. D	Osa28	315–421	6–8	Asu1	550–649	14–16	14.1	13.2
	B vs. D	Osa4	202–331	6–8	Msu1	494–621	14–16	12.3
7. 9–12 vs. 13–16	C vs. D	Vvi4	370–470	9–11	Ath9	602–706	13–15	10.3	10.2
	C vs. D	Pgi1	385–469	10–11	Ani11	606–689	14–15	10.1
8. 1–2 vs. 3–4	A	Cim2	104–162	1–2	Acl1	176–236	3–4	9.1	8.9
	A	Cim2	118–162	1–2	Pgu9	248–292	3–4	8.7
9. 5–6 vs. 7–8	B	Nfi3	251–291	5	Yli4	411–450	7	11.5	11
	B	Ani11	210–260	5	Tko1	244–294	7	10.5
10. 9–10 vs. 11–12	C	Sus2	351–394	9–10	Cco1	388–431	11–12	8.6	8.6
	C	Asu1	313–369	9–10	Pdi1	421–475	11–12	8.5

Entry 1 presents comparisons for the first eight-TMS half versus the second eight-TMS half. Entries 2–7 present comparisons for the four four-TMS quarters compared to each other. Entries 8–10 present comparisons for four representative adjacent 2 TMS hairpin structures