Table 1.
Comparison of genomic DNA isolated from Phytophthora and Pythium using the Kamiya and Kiguchi modified protocol and the DNeasy Plant Mini Kit (Qiagen)
| Species | Isolate | Kamiya and Kiguchi (2003) modified protocol | DNeasy Plant Mini Kit (Qiagen) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Conc. (μg/μl) | T.A. (μg)a | A260/A280 | A260/A230 | Conc. (μg/μl) | T.A. (μg)b | A260/A280 | A260/A230 | ||
| P. sojae | OH 1999 1.S.1.1 | 2.4 | 235 | 2.2 | 1.9 | 0.5 | 24 | 2.0 | 1.0 |
| OH 2000 Wood 25 | 2.1 | 207 | 2.2 | 1.9 | 0.6 | 33 | 2.0 | 1.3 | |
| OH 2000 Wood 31 | 1.5 | 145 | 2.1 | 1.7 | 0.5 | 23 | 1.9 | 1.1 | |
| OH SS05 | 1.3 | 133 | 2.2 | 1.8 | 0.3 | 13 | 2.1 | 1.0 | |
| MB 4-4-1 | 2.2 | 222 | 2.1 | 1.7 | 0.3 | 15. | 2.0 | 1.1 | |
| P. sansomeana | OH SS04 Defiance | 1.6 | 162 | 2.2 | 2.0 | 0.6 | 30. | 2.1 | 1.3 |
| OH Mad 245 | 2.4 | 242 | 2.2 | 2.1 | 0.8 | 39. | 2.2 | 1.3 | |
| OH SS05 3B-2 | 2.1 | 296 | 2.2 | 2.0 | 0.7 | 34. | 2.0 | 1.3 | |
| OH Brown 2312 | 2.9 | 285 | 2.2 | 2.1 | 0.9 | 43. | 2.2 | 1.4 | |
| P. heterothallicum | OH Blan B101-32 | 0.5 | 46 | 2.0 | 1.3 | 0.5 | 25. | 2.1 | 1.4 |
| OH Blan B101-41 | 1.8 | 182 | 2.2 | 2.1 | 0.8 | 40 | 2.2 | 1.3 | |
| OH Blan B101-31 | 2.4 | 243 | 2.3 | 2.0 | 0.7 | 350 | 2.1 | 1.2 | |
| OH Blan B408-14 | 2.3 | 234 | 2.3 | 2.1 | 0.7 | 35 | 2.1 | 1.4 | |
| OH Blan B101-22 | 0.5 | 53 | 2.1 | 1.4 | 0.5 | 26 | 2.0 | 1.5 | |
The DNA quantity and quality was determined by a NanoDrop ND-1000 spectrophotometer
aT.A. = total amount of DNA obtained in each sample diluted in 100 μl of TE Buffer
bTotal amount of DNA diluted in 50 μl of TB buffer