Figure 1.
DET1 dosage influences UV-C sensitivity upon recovery in both light and dark conditions. (A) mycDET1 overexpression in DET1 OE transgenic lines. Equal amounts of whole protein extracts from epitope-tagged mycDET1 lines OE-1, OE-2 and OE-3 were loaded on a 10% SDS–PAGE and analysed by immunoblot using an anti-myc antibody. The same blot was analysed with an anti-tubulin antibody as loading control. (B, C) Root growth inhibition upon UV-C exposure. Four-day-old seedlings with the indicated genotypes were exposed to UV-C (600 J/m2) and immediately returned to normal light conditions (B) or to complete darkness (C). Relative root growth was determined 24 h after irradiation by comparison with the respective non-irradiated control of the same genotype (100% root growth). Error bars represent standard deviations from three replicate experiments (n>20). Asterisks indicate t-test significant differences at P⩽0.05 relative to wild-type control at same dose.