| Threshold hydrophobicity of the H-segment |
n=3 (Dog pancreas RM), n=5 (yeast ER) in GGPG-nL/(19-n)A-GPGG H-segment |
n=3 (CoxVa), n=6 (Mgm1p) in GGPG-nL/(19-n)A-GPGG H-segment |
| Effect of Pro residues in the H-segment |
Ala → Pro replacements in the N-terminal three positions and the most C-terminal position decrease membrane insertion less than Ala → Pro replacements in the middle of the H-segment |
Qualitatively similar to the ER |
| Effect of aromatic residues in the H-segment |
Ala → Trp, Tyr replacements increase membrane insertion when near the N- or the C-terminus of the H-segment, but decrease insertion when in the middle of the H-segment |
Same as in the ER |
| Effect of positively charged residues in the H-segment |
Ala → Lys, Arg most unfavourable for membrane insertion when placed in the middle part of the H-segment |
Same as in the ER |
| Effect of negatively charged residues in the H-segment |
Ala → Asp, Glu most unfavourable for membrane insertion when placed in the middle part of the H-segment |
Ala → Asp, Glu most unfavourable for membrane insertion when placed in the middle and N-terminal (matrix-facing) part of the H-segment |
| Effect of negatively charged flanking residues |
DDPD… and EEPE… flanks decrease membrane insertion compared with GGPG… flank when placed next to the lumenal end of the H-segment |
DDPD… and EEPE… flanks decrease membrane insertion compared with GGPG… flank when placed next to the matrix end of the H-segment |
| Effect of positively charged flanking residues |
…KPKK and …RPRR flanks increase membrane insertion compared with …GPGG flank when placed next to the cytoplasmic end of the H-segment |
KKPK…KPKK and RRPR…RPRR flanks increase membrane insertion compared with GGPG…GPGG flanks when placed next to both the IMS and matrix ends of the H-segment |
