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. 2011 Feb 18;30(6):1093–1103. doi: 10.1038/emboj.2011.34

Figure 6.

Figure 6

Genetic regulatory interactions between tif1γ, gata1 and pu.1 in the PLM/ICM and EMP populations. Morpholinos were injected at the following concentrations: tifγ MO—1 ng/nl, gata1 MO2—5 ng/nl and pu.1 MO—20 ng/nl. (A) Expression of gata1 in wild-type and (B) tif1γ MO-injected embryos at 16 somites. (C) Expression of pu.1 in wild-type and (D) tif1γ MO-injected embryos at 16 somites. (E) Expression of scl in wild-type and (F) tif1γ MO-injected embryos at 16 somites. (G) gata1 expression in wild type, (H) gata1 morphants and (I) pu.1 morphants at 24 hpf. (J) pu.1 expression in wild type, (K) gata1 morphants and (L) pu.1 morphants at 24 hpf. (M) tif1γ expression in wild type, (N) gata1 morphants and (O) pu.1 morphants at 24 hpf. (P–W) Analysis of gata1 and pu.1 expression in the EMP population at 30 hpf in gata1- and pu.1 morphants. Expression of gata1 is similar in wild-type (P) and gata1 morphants (Q). (R) Expression of pu.1 in wild-type EMPs. (S) pu.1 expression is higher in gata1 morphants. Expression of gata1 in wild-type (T) and pu.1 morphants (U). (V) Expression of pu.1 in wild-type EMPs. (W) pu.1 expression is lost in pu.1 morphants. All embryos shown in lateral views, anterior to the left; numbers of embryos analysed are shown in each panel. See also Supplementary Figure S6.