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. 2011 Feb 18;30(6):1149–1161. doi: 10.1038/emboj.2011.35

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Copyright © 2011, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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ces-D acts on BR biosynthesis. (A) Illustration of the BR-biosynthetic pathway (according to Bishop (2007)), indicating changes in the ces-D mutant as compared with wild-type plants (for values see (B)). − denotes decreased; + denotes increased. (B) Endogenous BR levels of adult ces-D plants as compared with those of wild type. Aerial parts of 4-week-old plants were analysed for free BR levels (ng/g fresh weight). For each line, two independent sets of samples were measured and are shown. n.d., not detected (below the detection limit). (C) Semi-quantitative RT–PCR analysis of the expression of DWF4, CPD and ROT3 in 10-day-old ces-D and wild-type seedlings. UBQ5 was used as an internal control.