Figure 4.

Identification and characterization of a ces-ko line. (A) (Top) Schematic illustration of the ces-1 mutant. Coding regions are indicated as boxes. The arrow shows the predicted location of the T-DNA insertion. (Bottom) Semi-quantitative RT–PCR analysis of CES expression in 10-day-old seedlings of the ces-1 and those of wild-type Col-0. UBQ5 served as an internal control. (B) Response of ces-1 and ces-D seedlings to externally applied 24-epiBL and Brz2001. Seeds of ces-D, ces-1 and wild-type plants were germinated on medium supplemented with different concentrations of 24-epiBL or Brz2001 and incubated in 50 μmol/m²/s of continuous white light at 21±1°C for 7 days. Data points represent the average of 20 measured hypocotyls. Error bars show the s.e. (C) Response of DWF4, CPD and ROT3 expression in ces-1 and wild-type seedlings to external application of 24-epiBL or Brz2001 (performed as in (B)), analysed by quantitative real-time PCR. CDKA1 was used as an internal control.