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. 2011 Feb 18;30(6):1149–1161. doi: 10.1038/emboj.2011.35

Figure 5.

Figure 5

Generation and characterization of 35Sp:c-Myc-CES-SRDX plants. (A) Phenotype of plants constitutively expressing a c-Myc–CES–SRDX fusion protein. (Top) Seedlings grown in long-day conditions 5 DAG. (From left to right) Wild type and four independent homozygous lines transformed with a 35Sp:c-Myc-CES-SRDX construct. (Middle) Petiole length of 12-day-old seedlings in mm, measured in three replicates. (Bottom) Western blot analysis of the plants shown using an anti-c-Myc antibody. (B) Adult phenotypes of 35Sp:c-Myc-CES-SRDX/203 plants. Four-week-old plants of wild type, of an untreated 35Sp:c-Myc-CES-SRDX/203 plant, and of a 35Sp:c-Myc-CES-SRDX/203 plant treated with 24-epiBL are shown. (C) Quantitative real-time PCR analysis of the expression of DWF4, CPD and ROT3 in 2-week-old 35Sp:c-Myc-CES-SRDX ces-D and wild-type seedlings. UBQ5 was used as an internal control.