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. 2010 Oct 29;39(5):1763–1773. doi: 10.1093/nar/gkq1034

Figure 1.

Figure 1.

Purification and polymerization and exonuclease assays of human Pol ε. (A) Purified Exo+and Exo− human Pol ε was loaded onto a 10% SDS–polyacrylamide gel and stained with Coomassie. Molecular weight marker (MW) is shown with selected molecular weights indicated in kDa. (B) Image of DNA synthesis reaction products resolved on a 16% denaturing polyacrylamide gel. Lanes 2–5 are 1, 2, 5 and 10 min at 37°C with 1 nM Exo+Pol ε. Lanes 6–9 are 1, 2, 5 and 10 min at 37°C with 1 nM Exo− Pol ε. Lane 1 is a control reaction with no enzyme. (C) Image of 3′–5′ exonuclease reaction products resolved on a 16% denaturing polyacrylamide gel. Lanes 2–5 are 1.4, 2.8, 5.6 and 8.6 nM Exo+ Pol ε. Lanes 6–9 are 1.4, 2.8, 5.6 and 8.6 nM Exo− Pol ε. Reactions were performed as described in ‘Materials and Methods’ section. Template DNA sequences are shown to the side of each substrate.