Table 2.

Nucleotide binding

mADP
	rgyraKd (µM)	rgyr_Δlatch Kd (µM)
–	1.1 ± 0.3	0.16 ± 0.02
ssDNA	2.2 ± 0.4	0.67 ± 0.05
dsDNA	0.5 ± 0.2	0.13 ± 0.03
	rgyr_hel Kd (µM)	rgyr_hel_Δlatch Kd (µM)
–	4.4 ± 0.2	2.7 ± 0.1
ssDNA	10 ± 1	2.6 ± 0.7
dsDNA	1.7 ± 0.4	0.9 ± 0.4
ADPNP
	rgyr Kd (µM)	rgyr_Δlatch Kd (µM)
–	10 ± 0.8	4.7 ± 0.8
ssDNA	12 ± 3	12 ± 2
dsDNA	8 ± 1	8 ± 1
	rgyr_helbKd (µM)	rgyr_hel_ΔlatchbKd (µM)
–	36 ± 8	13 ± 4
ssDNA	12 ± 2	16 ± 8
dsDNA	1.9 ± 0.4	11 ± 4

^aData from ref. (11) .^bTitrations were performed with mADPNP.