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. 2010 Nov 4;39(5):1789–1800. doi: 10.1093/nar/gkq1048

Table 3.

DNA binding

rgyr
rgyr_Δlatch
Kd (µM) rel. Kd (µM) rel.
ssDNA
 – 0.017 + 0.004 1 0.033 ± 0.077 1
 ADP state 0.013 ± 0.003 0.8 0.025 ± 0.005 0.8
 ATP state (ADPNP) 0.015 ± 0.006 1.2 0.040 ± 0.010 1.2
dsDNA
 – 0.074 ± 0.016 4.3 0.096 ± 0.019 2.9
 ADP state 0.11 ± 0.02 6.5 0.058 ± 0.010 1.8
 ATP state (ADPNP) 0.055 ± 0.015 3.2 0.104 ± 0.015 3.2
rgyr_hela
rgyr_hel_Δlatch
Kd (µM) rel. Kd (µM) rel.
ssDNA
 – 0.20 ± 0.01 1 0.42 ± 0.07 1
 ADP state 0.28 ± 0.01 1.4 2.6 ± 0.3 6.2
 ATP state (ADPNP) 0.46 ± 0.03 2.3 1.1 ± 0.1 2.6
dsDNA
 – 3.9 ± 0.6 20 21 ± 5 50
 ADP state 3.7 ± 0.5 19 24 ± 5 57
 ATP state (ADPNP) 0.19 ± 0.03 1 36 ± 8 86

aData from ref. (11).