Table 3.
rgyr |
rgyr_Δlatch |
|||
Kd (µM) | rel. | Kd (µM) | rel. | |
ssDNA | ||||
– | 0.017 + 0.004 | 1 | 0.033 ± 0.077 | 1 |
ADP state | 0.013 ± 0.003 | 0.8 | 0.025 ± 0.005 | 0.8 |
ATP state (ADPNP) | 0.015 ± 0.006 | 1.2 | 0.040 ± 0.010 | 1.2 |
dsDNA | ||||
– | 0.074 ± 0.016 | 4.3 | 0.096 ± 0.019 | 2.9 |
ADP state | 0.11 ± 0.02 | 6.5 | 0.058 ± 0.010 | 1.8 |
ATP state (ADPNP) | 0.055 ± 0.015 | 3.2 | 0.104 ± 0.015 | 3.2 |
rgyr_hela |
rgyr_hel_Δlatch |
|||
Kd (µM) | rel. | Kd (µM) | rel. | |
ssDNA | ||||
– | 0.20 ± 0.01 | 1 | 0.42 ± 0.07 | 1 |
ADP state | 0.28 ± 0.01 | 1.4 | 2.6 ± 0.3 | 6.2 |
ATP state (ADPNP) | 0.46 ± 0.03 | 2.3 | 1.1 ± 0.1 | 2.6 |
dsDNA | ||||
– | 3.9 ± 0.6 | 20 | 21 ± 5 | 50 |
ADP state | 3.7 ± 0.5 | 19 | 24 ± 5 | 57 |
ATP state (ADPNP) | 0.19 ± 0.03 | 1 | 36 ± 8 | 86 |
aData from ref. (11).