Table 1.

Primers used in this study

Forward (F) and reverse (R) primers
Primers used with the template for the PUREsystem:
stop (F)	5′-TAAGAAGGAGATATACCAATGGTGCTTGGCAAACCGC-3′
stop (R)	5′-GTCTGCGCCACATCGGGGGAAACAAAATGG-3′
non-stop-1(R)	5′-CGCGTGCCGCCGTAAACGACGATGG-3′
non-stop-2 (R)	5′-GCGTGCCGCCGTAAACGACGATGG-3′
non-stop-3 (R)	5′-CGTGCCGCCGTAAACGACGATGG-3′
R4L4 (R)-Stop	5′-TATTCATTACAGCAGCAGCAGCAGCCTCCTCCTCCTACGAGTGCCGTAA-3′
R4L8 (R)-Stop	5′-TATTCATTACAGCAGCAGCAGCAGCAGCAGCAGCCTCCT-3′
1–140 (F)	5′-AAGGAGATATACCAATGATTGTGATTTCC-3′
1–140 (R)	5′-TATTCATTATTCCCGACCGCTG-3′
1–130 (R)	5′-TATTCATTACGCCTTCACGCTTG-3′
1–119 (R)	5′-TATTCATTACAGCCTGCGCTCTTTCG-3′
1–100 (R)	5′-TATTCATTATGTTGTTAATTCTTTAATCATATAGCCACCAGCCG-3′
universal	5′-GAAATTAATACGACTCACTATAGGGAGACCACAACGGTTT--CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCA-3′
Primers used for mutation of the yaeJ gene:
GGE (F)	5′-GGGCGCGGGCGGGGAGCATGTTAATAAGACC-3′
GGE (R)	5′-GGTCTTATTAACATGCTCCCCGCCCGCGCCC-3′
GAQ (F)	5′-GGGCGCGGGCGCGCAGCATGTTAATAAGACC-3′
GAQ (R)	5′-GGTCTTATTAACATGCTGCGCGCCCGCGCCC -3′
GAE (F)	5′-GGGCGCGGGCGCGGAGCATGTTAATAAGACC-3′
GAE (R)	5′-GGTCTTATTAACATGCTCCGCGCCCGCGCCC-3′
VAQ (F)	5′-TGCGCAGGGCGCGGTCGCGCAGCATGTTAAT-3′
VAQ (R)	5′-ATTAACATGCTGCGCGACCGCGCCCTGCGCA-3′