Figure 2.
Determination of DNA binding sensitivity of engineered ZFPs with the SEER–LAC system. The SEER–LAC signal was monitored using ZFP LacA-rrsA27 and rrsA62-LacB (A), ZFP LacA-rrsA125 and rrsA160-LacB (B) and ZFP LacA-rrsA1175 and rrsA1192-LacB (C) on the array at the different target DNA concentrations. Inset (A) represents digital image of nitrocefin assay after 30 min incubation.
