Table 1.

Sequences of zinc fingers and their intended DNA target sequences

^aThe most C-terminal zinc finger binds the 5′-end of the target site, therefore Finger 6 is shown to bind the first 5′-GAT-3′ of an 18-bp target site starting at position 27 of the rrsA gene.

^bAmino acids at positions −1, 1, 2, 3, 4, 5 and 6 of the α-helix of each finger are shown.

^cThe K_d-value of ZFP LacA-Zif268 was determined to be 7.7 nM under the conditions used in this assay. All values reported represent the results of at least two independent experiments and have a standard error of ±50%.

^dThe K_d-value of ZFP LacA-rrsA1175 could not be accurately determined under the conditions of this assay because the value was lower than the concentration of the DNA probe (55 pM).