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. 2010 Dec 9;39(5):e31. doi: 10.1093/nar/gkq1265

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Ligation scheme for constructing a 29-nt VS ribozyme substrate 23. (A) Phosphorylation of dinucleotide 18a with T4 PNK and ATP. (B) T4 RNA ligase-mediated ligation of phosphorylated dinucleotide 19 with 5′ flanking oligonucleotide 20 to yield 21. (C) Splint- and T4 DNA ligase-mediated ligation of 21 and 18-nt oligonucleotide 22 to yield full-length VS ribozyme substrate 23.