Figure 5.

Biochemical characterization of the second step ligation products for the HDV ribozyme substrate. Columns labeled ‘O’ contain wild-type substrate, while columns labeled ‘S’ contain 2′-O-o-NO₂-Bn/5′-S substrate. Substrates are labeled according to whether they were generated by the two-step ligation method (‘lig’) or by chemical synthesis (‘syn’). Lanes labeled ‘OH’ contain alkaline hydrolysis sequencing ladders, and lanes labeled ‘T1’ contain RNase T1 sequencing ladders. Samples exposed to UV light (100 W, 365 nm) for 4 min before analysis are labeled with a ‘+’ in the row labeled ‘UV’. Nucleotide sequences are indicated at the right of the gel. X = OH, O-o-NO₂-Bn; Y = O, S.