Gene expression of hyaluronan synthase isoforms (HAS1, HAS2, HAS3) in SMCs adhering on the different substrates with or without PDGF-BB (2 ng ml−1). Lane 1, TCP; lane 2, Fib; lane 3, ECM; lane 4, St; lane 5, TCP with PDGF-BB spiking; lane 6, Fib with PDGF-BB spiking; lane 7, ECM with PDGF-BB spiking; lane 8, St with PDGF-BB spiking. Ladder is shown on the left-hand side. GAPDH was used as the housekeeping gene.