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Purification of the HSV-1 UL28 protein. Protein samples from each major step in the purification procedure were analyzed by SDS/PAGE. Lane 1, initial cell lysate; lane 2, supernatant after lysis; lane 3, supernatant from wash of the insoluble material; lane 4, Ni-nitrilotriacetic acid agarose beads, after incubation with the solubilized protein fraction and extensive washing; lane 5, eluate, after dialysis; lane 6, Bio-Rad high-molecular-weight protein marker.
