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. 2001 Mar 13;98(6):3086–3091. doi: 10.1073/pnas.061555698

Figure 4.

Figure 4

(A) Localization of the binding site for UL28 protein. Comparison was made between UL28 protein binding to oligonucleotides bearing the 63-nt Ub upper strand (lanes 1 and 2) and binding to probes comprising a 31-nt nonconserved sequence component (non-p, lanes 3 and 4) and the 27-nt pac1 motif (lanes 5 and 6). DNA present at 5 nM was incubated in the absence or presence of 10 nM UL28 protein and complexes analyzed by EMSA. The slowly migrating DNA species formed by the pac1 oligonucleotide are denoted as pac1*. (B) Determination of the binding affinity of UL28 protein for pac1* DNA. The pac1 oligonucleotide, present at 5 nM, was incubated with increasing concentrations of UL28 protein (as noted), and complexes were visualized by EMSA.